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retrograde aav1 syn jgcamp8s wpre  (Addgene inc)


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    Addgene inc retrograde aav1 syn jgcamp8s wpre
    Retrograde Aav1 Syn Jgcamp8s Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav1+syn/pGEX-4T+MDM2+WT+(Plasmid+%2316237)/pmc13048257-294-7-9
    Average 96 stars, based on 176 article reviews
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    Related Articles

    Expressing:

    Article Title: Refinement of efficient encodings of movement in the dorsolateral striatum throughout learning.
    Article Snippet: .. To drive the expression of GCaMP6f in the striatum of either A2a or Drd1-Cre mice, we stereotaxically injected 400nL of AAV1-syn-Flex-GCaMP6f-SV40 (100833-AAV1, Addgene) into the dorsolateral striatum (from bregma, anterioposterior: 1.0mm; mediolateral: 2.25mm; and from dura, dorsoventral: − 2.45). ..

    Injection:

    Article Title: Refinement of efficient encodings of movement in the dorsolateral striatum throughout learning.
    Article Snippet: .. To drive the expression of GCaMP6f in the striatum of either A2a or Drd1-Cre mice, we stereotaxically injected 400nL of AAV1-syn-Flex-GCaMP6f-SV40 (100833-AAV1, Addgene) into the dorsolateral striatum (from bregma, anterioposterior: 1.0mm; mediolateral: 2.25mm; and from dura, dorsoventral: − 2.45). ..

    Article Title: “Enkephalinergic Neurons Gate Sex-Specific Control of Voluntary Micturition”
    Article Snippet: For anterograde axonal tracing studies, the majority of mice (≥ 2 for each mouse line representing the Bar population) received unilateral injections of AAV8-hSyn-DIO-mCherry (Addgene; plasmid #50459, 15-40nL). .. Additionally, for some of the pilot cases included here, mice were injected with AAV9-CAG-ChR2(H134R)-mCherry (UPenn; Addgene plasmid #100054), AAV1-Syn-Flex-GCaMP6s-WPRE-SV40 (UPenn; Addgene plasmid #100845), or AAV8-hSyn-DIO-hM3Dq(Gq)-mCherry (Addgene; plasmid #44361). .. For chemogenetic experiments, AAV8-hSyn-DIO-hM3D(Gq)-mCherry (Addgene; plasmid #44361, ∼40nL) was injected bilaterally for all mouse lines.

    Article Title: A cerebello-thalamo-cortical pathway transmits reward-based post-error signals for motor timing correction during learning in male mice.
    Article Snippet: .. From the next day to 3 days later, a mixture of AAV1-syn-flex-jGCaMP7f (1.2 × 1013 GC/mL; Addgene, #104492-AAV1) and AAV1-CAG-tdTomato (2.8–4.8 × 1011 GC/mL) was injected into the VL and VM (100 nL per site). .. For in vivo two-photon imaging of L5 neurons in M2, AAV1-syn-NES-jRGECO1a (1.7 × 1013 GC/mL; Addgene, #100854-AAV1) was injected into two sites in M2 (AP: 2.5mm, ML: 0.8mm,DV: 1.0mm; and AP: 2.0mm,ML: 0.8mm, DV: 1.0mm; 200 nL per site) the day after the head plate was attached.

    Article Title: Hippocampal input-driven plasticity of prefrontal interneurons reveals a circuit basis for impaired spatial working memory
    Article Snippet: .. AAV1.Syn::ChrimsonR-tdTomato (Addgene, 59171, 5x10 GC/mL) was injected into vHPC (D/V: -3.25, 600nL). .. A combination AAV9.CAG::FLEX.tdTomato (Addgene, 51503) and AAV9.EF1a::fDIO.EYFP (Vector Biolabs) with final titers of 1.7x10 and 1.25x10 GC/mL, respectively, was injected into mPFC (D/V: -1.45, 500 nL).

    Article Title: A cerebello-thalamo-cortical pathway transmits reward-based post-error signals for motor timing correction during learning in male mice
    Article Snippet: .. From the next day to 3 days later, a mixture of AAV1-syn-flex-jGCaMP7f (1.2 × 10 13 GC/mL; Addgene, #104492-AAV1) and AAV1-CAG-tdTomato (2.8–4.8 × 10 11 GC/mL) was injected into the VL and VM (100 nL per site). .. For in vivo two-photon imaging of L5 neurons in M2, AAV1-syn-NES-jRGECO1a (1.7 × 10 13 GC/mL; Addgene, #100854-AAV1) was injected into two sites in M2 (AP: 2.5 mm, ML: 0.8 mm, DV: 1.0 mm; and AP: 2.0 mm, ML: 0.8 mm, DV: 1.0 mm; 200 nL per site) the day after the head plate was attached.

    Plasmid Preparation:

    Article Title: “Enkephalinergic Neurons Gate Sex-Specific Control of Voluntary Micturition”
    Article Snippet: For anterograde axonal tracing studies, the majority of mice (≥ 2 for each mouse line representing the Bar population) received unilateral injections of AAV8-hSyn-DIO-mCherry (Addgene; plasmid #50459, 15-40nL). .. Additionally, for some of the pilot cases included here, mice were injected with AAV9-CAG-ChR2(H134R)-mCherry (UPenn; Addgene plasmid #100054), AAV1-Syn-Flex-GCaMP6s-WPRE-SV40 (UPenn; Addgene plasmid #100845), or AAV8-hSyn-DIO-hM3Dq(Gq)-mCherry (Addgene; plasmid #44361). .. For chemogenetic experiments, AAV8-hSyn-DIO-hM3D(Gq)-mCherry (Addgene; plasmid #44361, ∼40nL) was injected bilaterally for all mouse lines.

    Article Title: A subcortical switchboard for perseverative, exploratory and disengaged states.
    Article Snippet: .. For optogenetic stimulation, we used AAV9-hEF1a-DIO-mCherry-hChR2 (University of Zurich; V80-9, a gift from K. Deisseroth), AAV1-CAG-hChR2-tdTomato (virus made in the host institute, using Addgene plasmid #28017 a gift from K. Svoboda), AAV9-hSyn-SIO-FusionRed-stGtACR2 (virus made in the host institute, using Addgene plasmid #105677, a gift from O. Yizhar), AAV1-Ef1a-DIO-eNpHR3.0-EYFP (Addgene #26966-AAV1, a gift from K. Deisseroth), AAV1-hSyn-eNpHR3.0-EYFP (virus made in the host institute, using Addgene plasmid #26972, a gift from K. Deisseroth), AAV1-hSyn-DIO-ChrimsonR-tdTomato (virus made in the host institute), AAV1.Syn.ChrimsonR.tdTomato (Addgene #59171-AAV1, a gift from E. Boyden) and AAV1-CAG-tdTomato (Addgene #59462-AAV1, a gift from E. Boyden), for fibre photometry recordings, AAV1-Syn-flex-GCaMP6f (virus made in the host institute, using Addgene plasmid #100833, a gift from D. Kim and GENIE Project) and for retrograde tracing experiments, retroAAV-CAG-GFP and retroAAV-CAG-tdTomato (virus made in the host institute, using Addgene plasmids #37825 and #59462, gifts from E. Boyden). ..

    Virus:

    Article Title: A subcortical switchboard for perseverative, exploratory and disengaged states.
    Article Snippet: .. For optogenetic stimulation, we used AAV9-hEF1a-DIO-mCherry-hChR2 (University of Zurich; V80-9, a gift from K. Deisseroth), AAV1-CAG-hChR2-tdTomato (virus made in the host institute, using Addgene plasmid #28017 a gift from K. Svoboda), AAV9-hSyn-SIO-FusionRed-stGtACR2 (virus made in the host institute, using Addgene plasmid #105677, a gift from O. Yizhar), AAV1-Ef1a-DIO-eNpHR3.0-EYFP (Addgene #26966-AAV1, a gift from K. Deisseroth), AAV1-hSyn-eNpHR3.0-EYFP (virus made in the host institute, using Addgene plasmid #26972, a gift from K. Deisseroth), AAV1-hSyn-DIO-ChrimsonR-tdTomato (virus made in the host institute), AAV1.Syn.ChrimsonR.tdTomato (Addgene #59171-AAV1, a gift from E. Boyden) and AAV1-CAG-tdTomato (Addgene #59462-AAV1, a gift from E. Boyden), for fibre photometry recordings, AAV1-Syn-flex-GCaMP6f (virus made in the host institute, using Addgene plasmid #100833, a gift from D. Kim and GENIE Project) and for retrograde tracing experiments, retroAAV-CAG-GFP and retroAAV-CAG-tdTomato (virus made in the host institute, using Addgene plasmids #37825 and #59462, gifts from E. Boyden). ..

    Retrograde Tracing:

    Article Title: A subcortical switchboard for perseverative, exploratory and disengaged states.
    Article Snippet: .. For optogenetic stimulation, we used AAV9-hEF1a-DIO-mCherry-hChR2 (University of Zurich; V80-9, a gift from K. Deisseroth), AAV1-CAG-hChR2-tdTomato (virus made in the host institute, using Addgene plasmid #28017 a gift from K. Svoboda), AAV9-hSyn-SIO-FusionRed-stGtACR2 (virus made in the host institute, using Addgene plasmid #105677, a gift from O. Yizhar), AAV1-Ef1a-DIO-eNpHR3.0-EYFP (Addgene #26966-AAV1, a gift from K. Deisseroth), AAV1-hSyn-eNpHR3.0-EYFP (virus made in the host institute, using Addgene plasmid #26972, a gift from K. Deisseroth), AAV1-hSyn-DIO-ChrimsonR-tdTomato (virus made in the host institute), AAV1.Syn.ChrimsonR.tdTomato (Addgene #59171-AAV1, a gift from E. Boyden) and AAV1-CAG-tdTomato (Addgene #59462-AAV1, a gift from E. Boyden), for fibre photometry recordings, AAV1-Syn-flex-GCaMP6f (virus made in the host institute, using Addgene plasmid #100833, a gift from D. Kim and GENIE Project) and for retrograde tracing experiments, retroAAV-CAG-GFP and retroAAV-CAG-tdTomato (virus made in the host institute, using Addgene plasmids #37825 and #59462, gifts from E. Boyden). ..

    Imaging:

    Article Title: Brain region-specific gain modulation of place cells by VIP neurons.
    Article Snippet: .. We used AAV1-syn-flex-GCaMP6s (Addgene #100845) for imaging VIP cells, and AAV5-Syn-flex-rc[ChrimsonR-tdTomato] (Addgene, #62723) or AAV5-CAG-flex-ArchT-tdTomato (Addgene, #28305) for optogenetic modulation of VIP or SST cells. ..

    Article Title: Brain region-specific gain modulation of place cells by VIP neurons
    Article Snippet: .. We used AAV1-syn-flex-GCaMP6s (Addgene #100845) for imaging VIP cells, and AAV5-Syn-flex-rc[ChrimsonR-tdTomato] (Addgene, #62723) or AAV5-CAG-flex-ArchT-tdTomato (Addgene, #28305) for optogenetic modulation of VIP or SST cells. ..



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    Head-fixed mice learn to perform skilled forelimb movements to obtain a reward (A) Representation of injecting AAV9-hSyn-GRAB DA2h and AAV1-Syn-Flex-NES-jRGECO1a into M1. Coronal sections obtained from D1Cre and D2Cre mice showing expression of GRAB DA2h (green) and jRGECO1a (red) in the M1 forelimb area (brain slices are 0.5 mm anterior to the bregma). Scale bars, 500 μm. Magnification: 20×. See also for the histological map of GRAB DA2h and jRGECO1a expression and fiber tip placements. (B) Representation of the task in which head-fixed mice perform skilled forelimb movements with a joystick to obtain a delayed reward, and a fiber photometry setup for simultaneous recording of DA dynamics and population level Ca 2+ activity in the M1 forelimb area. See also for representative traces of neuronal Ca 2+ and DA signals acquired during training. (C) Increase in the number of rewarded joystick movements in D1Cre mice during training. (D) Movement kinematic parameters obtained from the early and late sessions in D1Cre mice showing that the average movement amplitude and velocity increased between training phases. (Left to right) Distribution of movement amplitudes, movement amplitude, and joystick velocity at the time of threshold crossing. (E) Individual movement trajectories of an example D1Cre mouse aligned to the initial joystick position (black dot), and preferred movement direction in the group of D1Cre animals (based on all extracted trajectories). (F) Change in movement similarity in D1Cre mice (based on the average distance between pairwise trajectories). (Left to right) Similarity between the early and late sessions; similarity within the late session. (G–J) Same as (C)–(F) for D2Cre mice. In (C)–(J), data were obtained from 14 D1Cre (10 Cre+ and 4 Cre-) and 13 D2Cre (10 Cre+ and 3 Cre-) animals. Data are represented as mean ± SEM. Paired t test; ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.

    Journal: iScience

    Article Title: The role of dopamine-sensitive motor cortical circuits in the development and execution of skilled forelimb movements

    doi: 10.1016/j.isci.2026.114983

    Figure Lengend Snippet: Head-fixed mice learn to perform skilled forelimb movements to obtain a reward (A) Representation of injecting AAV9-hSyn-GRAB DA2h and AAV1-Syn-Flex-NES-jRGECO1a into M1. Coronal sections obtained from D1Cre and D2Cre mice showing expression of GRAB DA2h (green) and jRGECO1a (red) in the M1 forelimb area (brain slices are 0.5 mm anterior to the bregma). Scale bars, 500 μm. Magnification: 20×. See also for the histological map of GRAB DA2h and jRGECO1a expression and fiber tip placements. (B) Representation of the task in which head-fixed mice perform skilled forelimb movements with a joystick to obtain a delayed reward, and a fiber photometry setup for simultaneous recording of DA dynamics and population level Ca 2+ activity in the M1 forelimb area. See also for representative traces of neuronal Ca 2+ and DA signals acquired during training. (C) Increase in the number of rewarded joystick movements in D1Cre mice during training. (D) Movement kinematic parameters obtained from the early and late sessions in D1Cre mice showing that the average movement amplitude and velocity increased between training phases. (Left to right) Distribution of movement amplitudes, movement amplitude, and joystick velocity at the time of threshold crossing. (E) Individual movement trajectories of an example D1Cre mouse aligned to the initial joystick position (black dot), and preferred movement direction in the group of D1Cre animals (based on all extracted trajectories). (F) Change in movement similarity in D1Cre mice (based on the average distance between pairwise trajectories). (Left to right) Similarity between the early and late sessions; similarity within the late session. (G–J) Same as (C)–(F) for D2Cre mice. In (C)–(J), data were obtained from 14 D1Cre (10 Cre+ and 4 Cre-) and 13 D2Cre (10 Cre+ and 3 Cre-) animals. Data are represented as mean ± SEM. Paired t test; ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05.

    Article Snippet: AAV1-Syn-Flex-NES-jRGECO1a , Addgene , RRID: Addgene_100853.

    Techniques: Expressing, Activity Assay